1. Absorption:1) Caco-2 permeability

2) Transmembrane transport test (Caco-2 drug transport test, P-gp drug transport test, BCRP drug transport test, OATs/0CTs/0ATPs, etc.)

2. Distribution:

1) Protein binding rate (plasma/tissue/microsome)

2) Whole blood/plasma allocation test

3. Metabolism:

1) Metabolic stability (liver microsomal stability test, S9 stability test, cell metabolic stability test, plasma and whole blood stability test)

2) Matrix stability: plasma, tissue and buffer

3) In vitro metabolite analysis and identification (metabolite prediction and confirmation; Metabolic pathway prediction and confirmation)

4. Drug-drug interactions:

1) Cytochrome P450(CYP) inhibition (IC50 and TDI)

2)P450 enzyme induction

Phenotypic analysis of enzymes :| and I enzymes (recombinant enzymes and chemical inhibition)

Physical and chemical properties (lipophilic, solubility test)

In vitro toxicity (hERG test, Mini-Ames)

Rapid screening